| 產品描述： | Z-IETD-FMK (Caspase-8 Inhibitor, Z-IE(OMe)TD(OMe)-FMK)是一種特異性Caspase-8抑制劑。Z-IETD-FMK也是顆粒酶B（granzyme B）抑制劑 |
| 靶點： |
Caspase-8;Caspase |
| 體外研究： |
Z-IETD-FMK, which inhibits the cleavage of caspase-8 and partially inhibits the cleavage of caspase-3 and PARP, prevents the execution of apoptosis in retinal cells exposed to different apoptotic stimuli. Z-IETD-FMK (50 μM) reduces ceramide-induced cardiomyocyte death and significantly inhibits the activation of caspase 3. Inhibition of caspase-8 by Z-IETD-FMK affects the generation of activated/memory T cells and T cell cytokine production, and decreases NF-kappaB responses to TCR:CD3 engagement in T cell cultures. |
| 體內研究： |
In vivo, inhibition of caspase-8 by Z-IETD-FMK reduces memory/activated CD4 and CD8 T cells, and increases susceptibility to T. cruzi infection. Z-IETD-FMK promotes neuronal survival and regeneration of injured retinal ganglion cells after CNS injuries. |
| 細胞實驗： |
Cell lines: Rat neonatal cardiomyocytes Concentrations: 50 μM Incubation Time: 24 h Method: Drugs were added to the cell culture medium simultaneously with ceramide, and surviving cells were counted 24 hours later. |
| 動物實驗： |
Animal Models: T. cruzi-infected mice Dosages: 0.4 mg/3 days Administration: -- |
| 參考文獻： |
1. Tezel G, et al. Inhibition of caspase activity in retinal cell apoptosis induced by various stimuli in vitro. Invest Ophthalmol Vis Sci. 1999, 40(11), 2660-2667. 2. Wang J, et al. Involvement of caspase 3- and 8-like proteases in ceramide-induced apoptosis of cardiomyocytes. J Card Fail. 2000, 6(3), 243-249. 3. Silva EM, et al. Caspase-8 activity prevents type 2 cytokine responses and is required for protective T cell-mediated immunity against Trypanosoma cruzi infection. J Immunol. 2005, 174(10), 6314-6321. 4. Monnier PP, et al. Involvement of caspase-6 and caspase-8 in neuronal apoptosis and the regenerative failure of injured retinal ganglion cells. J Neurosci. 2011, 31(29), 10494-10505. |
| 溶解性： |
soluble in DMSO |
| 保存條件： |
-20℃ |
| 配置溶液濃度參考： |
|
1mg |
5mg |
10mg |
| 1 mM |
1.527 ml |
7.637 ml |
15.275 ml |
| 5 mM |
0.305 ml |
1.527 ml |
3.055 ml |
| 10 mM |
0.153 ml |
0.764 ml |
1.527 ml |
| 50 mM |
0.031 ml |
0.153 ml |
0.305 ml |
|
| 注意： |
部分產品我司僅能提供部分信息，我司不保證所提供信息的權威性，僅供客戶參考交流研究之用。 |
上海工商
危險品化學品經營許可證（不帶存儲）
營業執照(三證合一)
北京